<big id="nokpt"><em id="nokpt"></em></big>

      ?
      生物資訊

      卓越性能,Mobitec巨大芽孢桿菌表達系統

      作者:admin 來源:本站 發布時間: 2019-04-16 19:06  瀏覽次數:
      購買進口儀器、試劑和耗材——就在始于2001年的畢特博生物 www.www.kabushidaigame.com

       

      性能參數

      產品名稱: 卓越性能,Mobitec巨大芽孢桿菌表達系統
      英文名稱: Bacillus megaterium Expression Systems
      產品型號: kit
      產品規格: kit
      產品產地: 德國
      品牌商標: MOBITEC
      價    格: 面議元
       

      產品詳細描述

       

      說明書(bmeg_handbook.pdf)

      Features:

      • Stable, high-yield protein expression in Bacillus megaterium 
         
      • Ideal for both small and large-scale protein production
      • Tightly regulated and efficiently inducible xylose operon (up to 350-fold)
      • No alkaline proteases activity even up to 5 hours after induction
      • No endotoxins observed
      • Versatile cloning (extended polylinker, additional BsrG I site*)
      • Versatile production (intracellular or extracellular)
      • Versatile purification (native, 6xHis-tag, Strep-tag, Strep/6xHis double-tag)
      • Removeable tag versions (for TEV or Xa proteases) available New!
      • Compatible with all B. subtilis vectors

      *BsrG I site is not included in all vectors.

      The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.

      B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

      High Protein Yield
      As a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.


      Versatile System with a Wide Range of Vectors
      MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.

      B. megaterium protoplasts specifically optimized for transformation 
      The protoplasts supplied by MoBiTec are from B. megaterium strain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies. 

      For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

      Examples:

      Selected Proteins successfully over-produced in Bacilli strains with our B. megaterium vectors:

      • β-Galactosidase (LacZ)1
      • Catabolite control protein (CcpA)2,3
      • Clostridium difficile toxin A4
      • Cobaltochelatase (CbiX)5
      • Dextransucrase6
      • Endolevanase (LevB)7
      • Glucose dehydrogenase (GdhA)1
      • Heat shock protein (HPr) from PTS (phosphotransferase sugar transport system)8
      • Human single-chain urokinase-like plasminogen activator (rscuPA)1
      • Levansucrase9
      • Mutarotase (Mro)1
      • Neopullulanase10
      • Translocation ATPase of the preprotein translocase (SecA)11
      • Trehalose repressor (TreR)12

       

       

      order#
      description
      amount
      BMEG02
      Bacillus megaterium protoplasts ready for transformation (strain WH320;1)
      Material is sufficient for 4 transformations plus control experiment.
      5 x 500 µl
      BMEG50
      Bacillus megaterium protoplasts, strain MS941
      5 x 500 µl
      BMEG31
      pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA
      10 μg
      BMEG32
      pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site
      10 μg
      BMEG30
      p3STOP1623hp, shuttle vector, lyophilized DNA
      10 μg
      BMEG33
      pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA
      10 μg
      BMEG34
      pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA
      10 µg
      BMEG03
      pWH1520 shuttle vector, original; lyophilized DNA
      5 µg
      BMEG10
      pMM1522 shuttle vector, improved; lyophilized DNA
      10 µg
      BMEG11
      pMM1525 shuttle vector with signal sequence; lyophilized DNA
      10 µg
      BMEG12
      pHIS1522 shuttle vector, 6xHis-tagged; lyophilized DNA precursor of BMEG20
      10 µg
      BMEG13
      pHIS1525 shuttle vector with signal sequence; 6xHis-tagged; lyophilized DNA
      10 µg
      BMEG14
      pSTREP1525 shuttle vector with signal sequence; Strep-tagged; lyophilized DNA
      10 µg
      BMEG15
      pSTREPHIS1525 shuttle vector with signal sequence;
      Strep/6xHis double-tagged; lyophilized DNA
      10 µg
      BMEG20
      pC-His1622 shuttle vector, C-term. 6xHis-tag; lyophilized DNA
      10 µg
      BMEG21
      pC-Strep1622 shuttle vector, C-term. Strep-tag; lyophilized DNA
      10 µg
      BMEG22
      pN-His-TEV1622 shuttle vector, N-term. His-tag,TEV-site
      10 µg
      BMEG23
      pN-Strep-TEV1622 shuttle vector, N-term. Strep-tag,TEV-site
      10 µg
      BMEG24
      pN-StrepXa1622 shuttle vector, N-term. Strep-tag; Xa site; lyophilized DNA
      10 µg
      BMEG25
      pSTOP1622, shuttle vector, lyophilized DNA
      10 µg
      BMEGT702
      Bacillus megaterium protoplasts, strain MS941, pretransformed with pT7-RNAP
      5 x 500µL
      BMEGT701
      Bacillus megaterium high yield T7 gene expression kit, 
      includes pretransformed protoplasts BMEGT702 (5 x 500µl), pPT7 cloning vector and pPT7-GFP control vector (vectors lyophilized, 10 µg each)
      Kit
      BMEGT710
      Bacillus megaterium pPT7 cloning vector, lyophilized
      10 µg
      shipped at RT, protoplasts shipped on dry ice 
      store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C
      All vectors are E. coli / B. megaterium shuttle vectors
      Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.

       

      請聯系我們

      客服QQ:1050524889

      郵箱:info@www.kabushidaigame.com

      電話:13366202681/010-82015225/400-833-9299

      網址:www.www.kabushidaigame.com

       

      購買進口儀器、試劑和耗材——就在始于2001年的畢特博生物 www.www.kabushidaigame.com

      所有試劑均用于科研 ???? 北京畢特博生物技術有限責任公司 版權所有
      服務熱線:010-82015225/400-833-9299 | 傳真:010-62015131 | E-mail:info@www.kabushidaigame.com
      京ICP備05028556號-1 ??京公網安備11010802008747??

        <big id="nokpt"><em id="nokpt"></em></big>

        男人把女人桶到高潮嗷嗷叫爽_娇妻被生人粗大猛烈进出高潮_乱人伦中文字幕在线_私密按摩师动漫在线观看